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141.
Disulfide bonds play an important role in stabilizing protein structure and regulating protein function. Therefore, the ability to infer disulfide connectivity from protein sequences will be valuable in structural modeling and functional analysis. However, to predict disulfide connectivity directly from sequences presents a challenge to computational biologists due to the nonlocal nature of disulfide bonds, i.e., the close spatial proximity of the cysteine pair that forms the disulfide bond does not necessarily imply the short sequence separation of the cysteine residues. Recently, Chen and Hwang (Proteins 2005;61:507-512) treated this problem as a multiple class classification by defining each distinct disulfide pattern as a class. They used multiple support vector machines based on a variety of sequence features to predict the disulfide patterns. Their results compare favorably with those in the literature for a benchmark dataset sharing less than 30% sequence identity. However, since the number of disulfide patterns grows rapidly when the number of disulfide bonds increases, their method performs unsatisfactorily for the cases of large number of disulfide bonds. In this work, we propose a novel method to represent disulfide connectivity in terms of cysteine pairs, instead of disulfide patterns. Since the number of bonding states of the cysteine pairs is independent of that of disulfide bonds, the problem of class explosion is avoided. The bonding states of the cysteine pairs are predicted using the support vector machines together with the genetic algorithm optimization for feature selection. The complete disulfide patterns are then determined from the connectivity matrices that are constructed from the predicted bonding states of the cysteine pairs. Our approach outperforms the current approaches in the literature. 相似文献
142.
We discuss numerical methods for simulating large-scale, integrate-and-fire (I&F) neuronal networks. Important elements in
our numerical methods are (i) a neurophysiologically inspired integrating factor which casts the solution as a numerically
tractable integral equation, and allows us to obtain stable and accurate individual neuronal trajectories (i.e., voltage and
conductance time-courses) even when the I&F neuronal equations are stiff, such as in strongly fluctuating, high-conductance
states; (ii) an iterated process of spike-spike corrections within groups of strongly coupled neurons to account for spike-spike
interactions within a single large numerical time-step; and (iii) a clustering procedure of firing events in the network to
take advantage of localized architectures, such as spatial scales of strong local interactions, which are often present in
large-scale computational models—for example, those of the primary visual cortex. (We note that the spike-spike corrections
in our methods are more involved than the correction of single neuron spike-time via a polynomial interpolation as in the
modified Runge-Kutta methods commonly used in simulations of I&F neuronal networks.) Our methods can evolve networks with
relatively strong local interactions in an asymptotically optimal way such that each neuron fires approximately once in
operations, where N is the number of neurons in the system. We note that quantifications used in computational modeling are often statistical,
since measurements in a real experiment to characterize physiological systems are typically statistical, such as firing rate,
interspike interval distributions, and spike-triggered voltage distributions. We emphasize that it takes much less computational
effort to resolve statistical properties of certain I&F neuronal networks than to fully resolve trajectories of each and every neuron within the system.
For networks operating in realistic dynamical regimes, such as strongly fluctuating, high-conductance states, our methods
are designed to achieve statistical accuracy when very large time-steps are used. Moreover, our methods can also achieve trajectory-wise accuracy when small time-steps are used.
Action Editor: Nicolas Brunel 相似文献
143.
New statistical modelling methods, such as neural networks (NNs), allow us to take a step further in the understanding of
complex relations in aquatic ecosystems. In this paper the results from the analysis of macro-invertebrate communities in
a complex riverine environment are presented. We attempted to explain observed changes in species composition and abundance
with neural network modelling methods and compared the results to linear regression. The NN method used is an improved form
of the RF5 algorithm, developed to effectively discover numeric laws from data. RF5 uses Product Unit Networks (PUNs), which
are in effect multivariate non-discrete power functions. The data set consisted of a 10-year time series of monthly samples
of macro-invertebrates on artificial substrates in the rivers Rhine and Meuse in the Netherlands. During this period the invertebrate
community has largely changed coinciding with the␣invasion of Ponto-Caspian crustaceans. We used physical–chemical data and
data on the abundance of the invasive taxa Corophium curvispinum and Dikerogammarus villosis to explain the observed changes in the resident invertebrate community. The analyses showed temperature, abundance of invasive
taxa and peak discharges as important factors. Comparison of the results from NN modelling to linear regression revealed that
the factors temperature and abundance of Dikerogammarus
villosis explained equally well in both cases. Only the neural network was able to use information on peak discharge and timing of
the peak in the previous winter to improve model performances. Neural networks are known to yield excellent modelling results,
a drawback however is their lack of transparency or their ‘black box’ character. The use of relatively easy interpretable
(white box) PUNs allows us to investigate the extracted relations in more detail and can enhance our understanding of ecosystem
functioning. Our results show that peak discharges might be an important factor structuring invertebrate communities in rivers
and hint on the existence of interacting effects from invasive species and discharge peaks. They finally show the value of
biological data sets that are collected over a long period and in a highly standardised way. 相似文献
144.
Temperature in agricultural production has a direct impact on the growth of crops. The emergence of greenhouses has improved the impact of the original unpredictable changes in temperature, but the temperature modeling of greenhouses is still the main direction at present. Neural network modeling relies on sufficient actual data
to model greenhouses, but there is a widening gap in the application of different neural networks. This paper
proposes a greenhouse temperature prediction model based on wavelet neural network with genetic algorithm
(GA-WNN). With the simple network structure and the nonlinear adaptability of the wavelet basis function,
wavelet neural network (WNN) improved model training speed and accuracy of prediction results compared with
back propagation neural networks (BPNN), which was conducive to the prediction and control of short-term
greenhouse temperature fluctuations. At the same time, the genetic algorithm (GA) was introduced to globally
optimize the initial weights of the original model, which improved the insensitivity of the model to the initial
weights and thresholds, and improved the training speed and stability of the model. Finally, simulation results
for the greenhouse showed that the model training speed, prediction results accuracy and model stability of
the GA-WNN in the greenhouse were improved in comparison to results obtained by the WNN and BPNN
in the greenhouse. 相似文献
145.
146.
相对于传统生化测定方法,基于近红外光谱(Near infrared spectroscopy,NIRS)玉米籽粒蛋白质含量检测是一种快速、非破坏、且适用于多组分同时检测的新方法。但在建模过程中,由于奇异数据(异常值)的存在会影响近红外光谱模型的预测精度和稳定性,我们采用奇异数据筛选法剔除了玉米籽粒近红外光谱中的奇异数据并建立了玉米籽粒蛋白质含量的偏最小二乘支持向量机(Least squares support vector machine,LS-SVM)模型。本文分别采用杠杆值法(Leverage)、半数重采样法(Resampling by Half-Mean,RHM)和蒙特卡洛采样法(Monte-Carlo Sampling,MCS)剔除了玉米籽粒蛋白质光谱数据中的奇异数据并对模型结果进行比较。在剔除奇异数据的基础上,采用偏最小二乘回归法(Partial least squares regression,PLSR)提取主成分,并基于小生境蚁群算法(Niche ant colony algorithm,NACA)优化偏最小二乘支持向量机(LS-SVM)模型参数(γ和σ2),建立基于LS-SVM的玉米籽粒蛋白质定量分析模型。结果表明,采用3种奇异数据筛选法剔除奇异数据后所建LS-SVM模型的预测结果都优于采用原光谱数据所建模型,相比较而言,蒙特卡洛采样法为基于近红外光谱检测玉米籽粒蛋白质的最佳奇异数据筛选法。 相似文献
147.
Mining impacts on stream systems have historically been studied over small spatial scales, yet investigations over large areas may be useful for characterizing mining as a regional source of stress to stream fishes. The associations between co-occurring stream fish assemblages and densities of various “classes” of mining occurring in the same catchments were tested using threshold analysis. Threshold analysis identifies the point at which fish assemblages change substantially from best available habitat conditions with increasing disturbance. As this occurred over large regions, species comprising fish assemblages were represented by various functional traits as well as other measures of interest to management (characterizing reproductive ecology and life history, habitat preferences, trophic ecology, assemblage diversity and evenness, tolerance to anthropogenic disturbance and state-recognized game species). We used two threshold detection methods: change-point analysis with indicator analysis and piecewise linear regression. We accepted only those thresholds that were highly statistically significant (p ≤ 0.01) for both techniques and overlapped within ≤5% error. We found consistent, wedge-shaped declines in multiple fish metrics with increasing levels of mining in catchments, suggesting mines are a regional source of disturbance. Threshold responses were consistent across the three ecoregions occurring at low mine densities. For 47.2% of the significant thresholds, a density of only ≤0.01 mines/km2 caused a threshold response. In fact, at least 25% of streams in each of our three study ecoregions have mine densities in their catchments with the potential to affect fish assemblages. Compared to other anthropogenic impacts assessed over large areas (agriculture, impervious surface or urban land use), mining had a more pronounced and consistent impact on fish assemblages. 相似文献
148.
149.
Genomic islands (GIs) are genomic regions that are originally transferred from other organisms. The detection of genomic islands in genomes can lead to many applications in industrial, medical and environmental contexts. Existing computational tools for GI detection suffer either low recall or low precision, thus leaving the room for improvement. In this paper, we report the development of our Ensemble algorithm for Genomic Island Detection (EGID). EGID utilizes the prediction results of existing computational tools, filters and generates consensus prediction results. Performance comparisons between our ensemble algorithm and existing programs have shown that our ensemble algorithm is better than any other program. EGID was implemented in Java, and was compiled and executed on Linux operating systems. EGID is freely available at http://www5.esu.edu/cpsc/bioinfo/software/EGID. 相似文献
150.
文章采用反向区间偏最小二乘法结合连续投影算法,筛选南丰蜜桔近红外检测的多元线性回归变量。对南丰蜜桔近红外光谱进行多元散射校正后,利用反向间隔偏最小二乘法,从500~1750 nm中初选出7个光谱区间,用于多元线性回归变量筛选。利用通过遗传算法和连续投影算法筛选出的变量建立了多元线性回归模型。经比较发现,利用反向区间偏最小二乘法结合连续投影算法筛选出的变量建立的多元线性回归模型,预测结果最优,模型预测相关系数为0.937,模型预测均方根误差为0.613 oBrix。结果表明,反向区间偏最小二乘法结合连续投影算法,可以有效地筛选近红外光谱的多元线性回归变量,提高南丰蜜桔可溶性固形物模型的预测精度。 相似文献